Fei Li, MD, PhD
Research Assistant Professor
Embryonic Stem Cells (ESC) and induced Pluripotent Stem Cells (iPSC) are promising source of more than 200 types of adult tissue cells, including vascular endothelial cells. Contrasting to adult tissue stem cells, (i.e. bone marrow stem cells or other stem cells) ESCs and iPSCs can be maintained in the laboratory for extended period of time without losing their stemness. However, the mechanisms of controlling the differentiation of ESCs and iPSCs are less known. Ongoing research in my laboratory addresses the subject of regulated differentiation in the context of generating functional vascular precursors and endothelial lineages. One biological property of ESCs and iPSCs is, when differentiated in specific conditions, the onset and development of lineage-specific tissue cells mirror the events in developing embryos. Using this to our advantage experiments are designed to delineate extracellular signals involved in the onset and differentiation of vascular progenitors for endothelial and vessel mural cells. Purified vascular progenitor fractions derived from ESCs and iPSCs are characterized for their lineage markers, genetic makeup, in vitro and in vivo vasculogenic and angiogenic activities. Further the vaso-protective efficacy of these vascular progenitors is put to test in mice modeling bacteria LPS-induced acute lung injury (ALI), an acute and lethal disease due to the breakdown of lung micro-vascular barrier.
Fei Li, M.D., Ph.D.
Research Assistant Professor
Department of Pharmacology
University of Illinois at Chicago
Toya S, Li F, Bonini MG, Gomes I, Mao M, Bachmaier KW, Malik AB. Interaction of a Specific Population of Human Embryonic Stem Cell–Derived Progenitor Cells with CD11b+ Cells Ameliorates Sepsis-Induced Lung Inflammatory Injury American Journal of Pathology 178: 313-324, 2011
Lu SJ, Feng Q, Ivanova Y, Luo C, Li T, Li F, Honig GR, Lanza R. Recombinant HoxB4 fusion proteins enhance hematopoietic differentiation of human embryonic stem cells. Stem Cells Dev. 16: 547 559, 2007.
Li F, Lu SJ, Honig GR. Hematopoietic cells from primate embryonic stem cells. Methods Enzymol. 418: 243-251, 2006.
Lu SJ, Li F, Vida L, Honig GR. CD34+CD38- hematopoietic precursors derived from human embryonic stem cells exhibit an embryonic gene expression pattern. Blood 103: 4134-4141, 2004.
Lu SJ, Li F, Vida L, Honig GR. Comparative gene expression in hematopoietic progenitor cells derived from embryonic stem cells. Experimental Hematology 30: 58-66, 2002.
Lu SJ, Quan CG, Li F, Vida L, Honig GR. Hematopoietic progenitor cells derived from embryonic stem cells: analysis of gene expression. Stem Cells 20: 428-437, 2002.
Li F, Lu S, Vida L, Thomson JA, Honig GR. Bone morphogenetic protein 4 induces efficient hematopoietic differentiation of rhesus monkey embryonic stem cells in vitro. Blood 98: 335-342, 2001.